Description:
IsoAmpIIUniversaltHDAkitcanbeusedtoamplifyanddetectshortDNAsequences(70bp-120bp)ataconstanttemperature.Thekitcanbeusedwithavarietyoftemplates,includingmicrobialgenomicDNA,viralDNA,plasmidDNA,andCDNA.AsinglecopyoftargetDNAcanbeamplifiedbytHDAanddetectedbyagarosegelelectrophoresiswhenoptimizedprimersandbufferareused.
IsoAmpIIUniversaltHDAkitisbasedonasecond-generationThermophilicHelicase-DependentAmplificationplatform.ThereactionssupportedbyIsoAmp®IIUniversaltHDAkitincludetHDA,reversetranscriptionHDA(RT-HDA),real-timequantitativeHDA(qHDA)andreal-timequantitativeRT-HDA(qRT-HDA),fromasinglereactionbuffer.
IsoAmpIIUniversaltHDAkitcontainsanenzymemix,dNTPsolutionandbuffer,whichallowsflexibilityinreactionsetup.AControlTemplateandasetofspecificprimersaresuppliedforthepositivecontrolreaction.
Figure1.HDATechnology.
Figure2.PositivecontrolassayswithvaryingamountofControlTemplate.
Positivecontrolassayswereconductedfollowingtheone-stepqHDAprotocol.ControlTemplatewasusedat5ng-500fgperassayintriplicate.
*Negativecontrolreactionsmayproduceprimer-dimer,whichcanbedistingushedfromspecificampliconsbygel-analysisorbymelting-curveanalysis.
Applications:
UsedtoamplifyanddetectshortDNAsequences(80-120bp)
-DownloadManual
Advantages:
UseofhelicasetoseparateDNAeliminatesneedforthermocyclerReactionscanbeperformedatconstanttemperature.Workswithavarietyoftemplates,includingbacterialgenomicDNA,viralDNA,plasmidDNAandcDNA.AslowasasinglecopyoftargetDNAcanbeamplifiedunderoptimizedconditions.Canbeusedforreal-timequantitativeor/andreversetranscriptionHDA.
KitComponents:
IsoAmp®EnzymeMix(50reactions)
IsoAmp®dNTPSolution(50reactions)
10XAnnealingBufferII
MgSO4(100mM)
NaCl(500mM)
pCNG1(ControlTemplate:1ng/µl)
NGF3(ForwardPrimer:5µM)
NGR3(ReversePrimer:5µM)
Note:ControlTemplateandPrimershavebeenchanged.
StorageConditions:
StorageTemperature:-20ºC
RecommendedstorageconditionsforthetHDAkitare-20°Cforshort-term(lessthan6months)storageand-80°Cforstoragegreaterthan6months.
QualityControl:
QualityAssuranceStatement:
KitcomponentswereindividuallytestedforoptimalperformanceintHDA.
Legal:
NOTICETOPURCHASER:ThisproductiscoveredbyU.S.patents7,282,328and7,662,594andpendingforeignpatents.Thepurchasepriceofthisproductincludesalimited,non-transferablelicenseforresearchuseonlyunderthepatentsownedbyBiohelix.Nootherlicenseunderthesepatentsisconveyedexpresslyorbyimplicationtothepurchaserbythepurchaseoftheproduct.TheProductisnottobeusedfordiagnosticpurposesnorisitintendedforhumanuse.Thisproductmaynotberesold,modifiedforresale,orusedtomanufacturecommercialproductswithoutwrittenapprovalofBioHelixCorp.FurtherinformationregardingthisproductmaybeobtainedbycontactingBioHelixatinformation@biohelix.com.
